Next-generation fluorescent probes for the detection of protein unfolding

Project No.
lzp-2024/1-0330
Project coordinator
Latvian Institute of Organic Synthesis (LIOS)
Project leader
Dr. Teodors Panteļejevs (LIOS)
Implementation period
36 months (2025-2027)
Total cost
300 000,00 EUR

The aim of this project is to obtain improved protein-sensitive fluorescent dyes that can make the application of differential scanning fluorimetry more robust and widely implementable.

During the project, new reagents with improved properties for biophysical experiments will be developed. The aim is to design fluorescent dyes for use in differential scanning fluorimetry (DSF) measurements. The synthesis will focus on compounds based on the widely used SYPRO Orange dye. Compounds with various substituent groups will be tested in DSF assays to determine structure-function relationships, ultimately leading to an optimized compound with high solubility, brightness, and protein-binding affinity.

The obtained reagents have high potential for application in drug development. The developed dyes can be used for identifying new drug candidates using the target-based drug discovery approach. Such reagents can accelerate the drug discovery process, reduce its costs, and facilitate the identification of high-quality drug candidates.