Prof. Dr. habil. biol. Nikolajs Sjakste
Biochemistry Team is organized following pattern of a mixed research unit, its staff work in co-operation with Department of Medical Biochemistry of the University of Latvia and Genomics and Bioinformatics Team of Institute of Biology of the University of Latvia. Premises of the Team are outside main building of the Institute, the team is housed downtown in the building of the Faculty of Biology of the University of Latvia. Main branches of the research: mechanism of action of the drugs developed in LIOS, role of nitric oxide in mechanism of action of pharmacological remedies, DNA-drug interactions. In recent years scientific interest of the team is focused on search for remedies for treatment of diabetes mellitus and its complications. Substances with both DNA-protecting and antioxidant activities are sought. Exploration of the direct interaction of ftorafur with DNA and mechanism of interaction of antimutagenic 1,4-dihydropyridine with DNA are our recent achievements.
Selected recent publications
- Ošiņa K, Rostoka E, Sokolovska J, Paramonova N, Bisenieks E, Duburs G, Sjakste N, Sjakste T. 1,4-Dihydropyridine derivatives without Ca2+-antagonist activity up-regulate Psma6 mRNA expression in kidneys of intact and diabetic rats. Cell Biochem. Funct. 2016; 34 (1), 3-6.
- Ošiņa K, Rostoka E, Isajevs S, Sokolovska J, Sjakste T, Sjakste N. Effects of an antimutagenic 1,4-dihydropyridine AV-153 on expression of nitric oxide synthases and DNA repair-related enzymes and genes in kidneys of rats with streptozotocin model of diabetes mellitus. Basic Clin. Pharmacol Toxicol. 2016 (accepted for publication)
- Leonova E, Sokolovska J, Boucher JL, Isajevs S, Rostoka E, Baumane L, Sjakste T, Sjakste N. New 1,4-Dihydropyridines Down-regulate Nitric Oxide in Animals with Streptozotocin-induced Diabetes Mellitus and Protect DNA against Peroxynitrite Action. Basic Clin. Pharmacol. Toxicol. 2015 Dec 12. doi: 10.1111/bcpt.12542. [Epub ahead of print]
- Sokolovska J, Isajevs S, Rostoka E, Sjakste T, Trapiņa I, Ošiņa K, Paramonova N, Sjakste N. Changes in glucose transporter expression and nitric oxide production are associated with liver injury in diabetes. Cell Biochem Funct. 2015, 33(6), 366-74.
- Buraka E, Chen CY, Gavare M, Grube M, Makarenkova G, Nikolajeva V, Bisenieks I, Brūvere I, Bisenieks E, Duburs G, Sjakste N. DNA-binding studies of AV-153, an antimutagenic and DNA repair-stimulating derivative of 1,4-dihydropiridine. Chem Biol Interact. 2014, 220, 200-207.